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Reverse Transcription PCR

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Explain why ribonucleic acid (RNA) targets need reverse transcription before polymerase chain reaction (PCR) amplification.

Intermediate8 min read15 sectionsMolecular Diagnostics
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Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Define reverse transcription polymerase chain reaction (RT-PCR) as a method for ribonucleic acid (RNA) targets
  • Explain that reverse transcriptase converts RNA to complementary DNA (cDNA) before PCR
  • State why RNA assays still follow the same clinical workflow and validated interpretation rules

Section 1 of 15