Overview
Dengue IgM/IgG immunoassays detect antibody responses to dengue virus structural antigens.
Clinical Importance
Diagnose dengue after the early NS1 window
Support primary vs secondary infection pattern assessment
Specimen Requirements
- Specimen: serum (preferred for most serology/autoimmune immunoassays) or plasma when the method is validated
- Tube: gold/SST (serum separator) or red-top serum; follow method-specific plasma acceptability
- Handling: allow SST to clot fully; centrifuge and separate promptly; avoid repeated freeze–thaw
- Storage: refrigerate short-term; freeze aliquots for delayed testing per manufacturer stability
- Stability: analyte- and kit-specific; do not exceed validated time/temperature limits
Analytical Method
Capture IgM and IgG ELISA/rapid tests. Cross-reactivity with other flaviviruses (Zika, yellow fever, JE, vaccine) complicates interpretation. PRNT and PCR refine difficult cases.
Instruments Used
Automated analyzers
- ELISA processors for dengue antibodies
- Roche Cobas immunoassay systems
- Abbott Alinity / Architect immunoassay systems
- Siemens Healthineers Atellica / ADVIA Centaur immunoassay systems
Manual methods
- Rapid dengue IgM/IgG cassettes
Reference Values
Assay cutoffs, units, and reference intervals are method- and manufacturer-specific. Always interpret against the performing laboratory report (CLSI EP28-A3c for quantitative intervals where applicable). Serologic patterns require clinical correlation and current guideline algorithms (WHO/CDC/society guidance as adopted locally).
Nonreactive expected without recent/past dengue or cross-reactive flavivirus exposure. Indices are kit-specific.
Interpretation
Common Causes of Increased Results
- IgM reactive; supports recent dengue/flavivirus infection with clinical correlation
- High IgG ± IgM patterns; may suggest secondary infection depending on algorithm
Common Causes of Decreased Results
- Seronegative early in illness; repeat or use NS1/PCR
Clinical Significance
- Dengue laboratory diagnosis algorithms
Factors Affecting Results
Pre-analytical
- Hemolysis, lipemia, or icterus (assay-dependent interference)
- Wrong tube type or incomplete clotting of SST
- Repeated freeze–thaw of serum aliquots
- Testing too early or too late relative to infection/vaccination timing for serology
Analytical
- Calibration/QC failure; reagent lot shifts
- Hook effect / antigen excess on selected immunoassays
- Heterophile antibodies, rheumatoid factor, or biotin interference (assay-dependent)
Post-analytical
- Applying another laboratory’s cutoff to a different method
- Interpreting a single serology result without clinical timing and confirmatory algorithms
Advantages and Limitations
Advantages
- Useful after viremic/NS1 phase
Limitations
- Flavivirus cross-reactivity
- Rapid tests vary in performance
Clinical Pearls
Dengue IgM in a flavivirus-vaccinated traveler needs extra humility; cross-reaction is real.
References
Authoritative textbooks, guidelines, and reviews supporting this laboratory test reference. Verify reference intervals, critical limits, and method details against institutional protocols and current guideline editions.
Textbooks
- McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods (verify current edition). Chapters on clinical immunology and serology.
- Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics (verify current edition). Immunoassay and protein chapters.
CLSI and Professional Guidelines
- Centers for Disease Control and Prevention. Laboratory testing recommendations for infectious disease serology and related algorithms (verify current CDC guidance for the pathogen).
- Clinical and Laboratory Standards Institute. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory. CLSI document EP28-A3c (verify current edition).
- Clinical and Laboratory Standards Institute. Immunoassay and related method evaluation documents (e.g., EP05, EP06, EP07, EP17; verify current editions relevant to the assay).
- College of American Pathologists. Accreditation and proficiency testing expectations for immunology/serology (verify current CAP checklist requirements).
- International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). Recommendations relevant to protein and immunoassay measurands (verify current IFCC documents).
WHO Publications
- World Health Organization. Laboratory and diagnostic guidance for serologic and immunologic testing (verify current WHO publication for the analyte/assay).
Frequently Asked Questions
Common questions about using this Dengue IgM / IgG laboratory test Medical Laboratory Library reference in Medical Laboratory Science education.
What is the Dengue IgM / IgG laboratory test reference used for?
This Medical Laboratory Library page summarizes the clinical importance of Dengue IgM / IgG. Review the Clinical Importance section for why the test is ordered in laboratory medicine practice.
Where can I find Dengue IgM / IgG specimen requirements?
See the Specimen Requirements section on this page for specimen information related to Dengue IgM / IgG. Confirm collection details against your laboratory protocol.
Where are Dengue IgM / IgG reference values listed?
See the Reference Values section on this Laboratory Library page. Reference ranges can vary by method and population, so verify intervals with your laboratory.
How do I interpret Dengue IgM / IgG results?
Use the Interpretation section for laboratory interpretation framing, including increased and decreased result patterns when provided. Do not treat educational text as a patient-specific diagnosis.
What analytical method is used for Dengue IgM / IgG?
See the Analytical Method section for principle and method notes. Method details may differ by instrument and institutional SOP.
Are related laboratory tests linked from Dengue IgM / IgG?
Yes. The Related Laboratory Tests section lists connected references for continued lookup in the Medical Laboratory Library.
How can I continue learning after reading Dengue IgM / IgG?
Use Continue learning links on this page for related Academy lessons, medical laboratory quizzes, or laboratory case studies when available for this Immunology & Serology topic.
What are the clinical pearls for Dengue IgM / IgG?
See the Clinical Pearls section for concise laboratory practice notes associated with Dengue IgM / IgG. Apply pearls alongside institutional protocols.
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