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The PCR Cycle

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Describe denaturation, annealing, and extension as practical polymerase chain reaction (PCR) cycle steps.

Intermediate8 min read15 sectionsMolecular Diagnostics
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Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Name the three practical polymerase chain reaction (PCR) cycle steps: denaturation, annealing, and extension
  • State what each step accomplishes in plain laboratory language
  • Explain why repeated cycles increase copies of the selected target for detection

Section 1 of 15