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Multiplex PCR

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Explain multiplex polymerase chain reaction (PCR) as detecting more than one target in a single reaction when the assay is designed for it.

Intermediate7 min read15 sectionsMolecular Diagnostics
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Learning Objectives

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Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Define multiplex polymerase chain reaction (PCR) as amplifying and detecting more than one selected target in one reaction when the assay is designed for it
  • Explain why multiplex panels must be interpreted target-by-target under the assay standard operating procedure (SOP)
  • Recognize that combining unrelated singleplex assays in one tube is not the same as a validated multiplex assay

Section 1 of 15