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Fluorescence and Detection

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Describe fluorescence detection in real-time polymerase chain reaction (PCR) in simple laboratory terms.

Intermediate8 min read15 sectionsMolecular Diagnostics
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Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Explain that many real-time polymerase chain reaction (PCR) assays use a fluorescence signal to detect amplification as it happens
  • Relate rising fluorescence to target amplification under assay-defined detection chemistry
  • State that signal interpretation, including cycle threshold (Ct) / quantification cycle (Cq) calls, follows the validated assay standard operating procedure (SOP)

Section 1 of 15