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Indirect Antiglobulin Test (IAT)

This Medical Laboratory Science lesson in Blood Bank supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Apply the indirect antiglobulin test principle, in vitro sensitization followed by AHG detection, across antibody screening, identification, crossmatch, and antigen typing, with attention to the check-cell control.

Intermediate28 min read15 sectionsBlood Bank
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Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective below describes a competency you will apply at the immunohematology bench, practical laboratory reasoning first, memorization second.

  • Explain the two-step indirect antiglobulin test principle: in vitro incubation to allow antibody sensitization, followed by AHG addition to detect that sensitization
  • Identify the major applications of the IAT principle in blood bank practice, antibody screening, antibody identification, AHG crossmatch, and some antigen typing methods
  • Interpret AHG (check cell) control results and explain why a negative screen/panel/crossmatch is only valid when the check-cell control performs as expected

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